Figure 4. NMR reveals differences in the PH domain dependent on C-tail phospho states.

(A) Overlay of ¹⁵N-¹H HSQC spectra of the PH domain in the context of full-length semisynthetic Akt with non-phosphorylated C-tail (blue) and pSer473 (red). Select residue-specific assignments are shown. (B) Overlay of ¹⁵N-¹H HSQC spectra of the PH domain in the context of full-length semisynthetic Akt with non-phosphorylated C-tail (blue) and pSer477/pThr479 (green). Select residue-specific assignments are shown. (C) Expanded spectra around the peaks assigned to Ile6, Gln43, Asp44, Thr65 and Thr101. Overlay of all three spectra, same color coding. Arrows indicate chemical shift perturbations. Note that contour levels have been plotted lower for the non-p-C Gln43 and pSer477/pThr479 Asp44 peaks.