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. 2020 May 4;100(9):1197–1207. doi: 10.1038/s41374-020-0436-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Top panels: double immunofluorescence staining of insulin (green fluorescence) and MANF (red fluorescence) on pancreatic tissue sections from β cell-specific Wfs1 knockout mice (βWfs1^(−/−)) taken 5 weeks after intraperitoneal injection of AAV-control (AAV9-CBA-IRES-GFP) or AAV9-MANF (AAV9-CBA-MANF-IRES-GFP) vector (n = 6 each group). Middle panels: double immunofluorescence staining of insulin (green fluorescence) and Ki67 (red fluorescence) of pancreatic sections from βWfs1^(−/−) mice taken 5 weeks after intraperitoneal injection of AAV-control or AAV9-MANF vector (n = 6 in each group). Bottom panels: double immunofluorescence staining of insulin (green fluorescence) and TUNEL staining (red fluorescence) of pancreatic sections from βWfs1^(−/−) mice taken 5 weeks after intraperitoneal injection of AAV-control or AAV9-MANF vector (n = 6 each group). The magnification is ×10 for each image, and ×40 for the right upper images. b Quantification of Ki67-positive β cells in AAV-control or AAV9-MANF injected βWfs1^(−/−) mice (AAV-control, n = 5; AAV9-MANF, n = 6; *P < 0.05). c Quantification of β cell mass in AAV-control or AAV9-MANF injected βWfs1^(−/−) mice (n = 6 in each group, not significant).