Extended Data Fig. 8. Transfer of young eosinophils is associated with alterations in muscle stem cell frequencies but not function.

(a) Gating strategy and representative flow plots of CD31^–, CD45^–, Sca-1^–, Vcam⁺ and integrin α7⁺ satellite cells in muscle of Young (n=13), Aged-PBS (n=17), Aged-yEOS, (n=17) and Aged-yEOS^{IL−4−/−} (n=17) mice. (b) Quantification of muscle stem cell frequencies in indicated groups (c) Representative photographs of immunofluorescent stained sort-purified and differentiated satellite cells. (d) Quantification of cell colony formation of sort-purified muscle stem cells of Young (n=10), Aged-PBS (n=10), Aged-yEOS (n=8) and Aged-yEOS^{IL−4−/−} (n=8) mice (e) Representative H&E stained longitudinal and cross-sectional quadriceps femoris in indicated groups. (f) Quantification of centrally nucleated myofibers in sections of Young (n=26), Aged-PBS (n=26), Aged-yEOS (n=18) and Aged-yEOS^{IL−4−/−} (n=13) mice. (g) Muscle weight (femur) was measured in Young (n=5), Aged-PBS (n=9), Aged-yEOS (n=8) and Aged-yEOS^{IL−4−/−} (n=7) mice. Data (a-f) are pooled from 2 independently performed experiments except for g (one experiment has been performed). Statistical significance was calculated by one-way ANOVA followed by two-tailed post-hoc Dunnett’s multiple comparison test against the aged-PBS treated group. Data are shown as individual data points with mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001.