Extended Data Fig. 9. Eosinophil transfers reverse myeloid skewing in old age.
(a) Gating strategy for the identification of LSK, HSC-SLAM, HSC-AD, CMP, CLP and GMP populations. (b) Absolute numbers of CMP, CLP and GMP per mouse in Young (n=5), Aged-PBS (n=7) and Aged-yEOS (n=8) groups. (c) Absolute numbers of CMP, CLP and GMP per mouse in Aged-PBS (n=8) and Aged-yEOSIL−4−/− (n=10) groups. One out of two independently performed experiments is shown and data are shown as individual data points with mean ± SEM. Statistical significance in (b) was calculated one-way ANOVA followed by two-tailed post-hoc Dunnett’s multiple comparison test against the aged-PBS treated group and (c) by two-tailed Student’s t-test. (d) Gating strategy for the identification of germinal GCB. Data are shown as individual data points with mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001.