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. 2020 Aug 19;10:14014. doi: 10.1038/s41598-020-70859-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Size of PSD95 puncta, asymmetric synapses and symmetric synapses. (a) Area of PSD95-positive puncta (µm²) acquired by SDM in the hippocampus (CA1) (Mean + SD). Asterisks indicate statistically significant differences. (b, c) Mean size of the synaptic apposition surface (SAS) of asymmetric and symmetric synapses (µm²) estimated from stacks of serial sections acquired by FIB-SEM from the same region. See also Table 2.