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. 2020 Aug 19;10:14014. doi: 10.1038/s41598-020-70859-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Frequency histograms of the sizes of PSD95 puncta and asymmetric synapses. (a–c) Comparison of the distribution of the surface areas of PSD95 puncta acquired by SDM (red line) and the synaptic apposition surfaces of asymmetric synapses reconstructed from FIB-SEM samples (blue line) from three layers of the CA1 region of the hippocampus. The histograms corresponding to asymmetric synapses are narrower and lie to the left of the histograms corresponding to PSD95 puncta. (d–f) Frequency histograms (blue bars) of the areas of the synaptic apposition surfaces of asymmetric synapses reconstructed from FIB-SEM samples. The log-normal distributions (black lines) represent the theoretical probability density functions that best fit the experimental data. The parameters µ and σ of the corresponding log-normal distributions have also been indicated.