Table 1.
Relationship between the densities of puncta and the densities of synapses.
| Stratum of CA1 | Density of PSD95 (puncta/100 µm2) | Density of SAP102 (puncta/100 µm2) | Colocalization index | Total density of puncta/100 µm2 | Density of AS (synapses/µm3) | Conversion factor |
|---|---|---|---|---|---|---|
| Lacunosum-Moleculare | 91.3103 ± 13.3605 | 77.1788 ± 23.8497 | 0.5840 | 106.4372 ± 17.6450 | 1.5958 ± 0.7317 | 0.0150 |
| Radiatum | 118.4513 ± 25.8022 | 122.7022 ± 24.2477 | 0.6633 | 145.1760 ± 25.6250 | 2.3076 ± 0.3788 | 0.0159 |
| Oriens | 119.7759 ± 21.1004 | 115.6872 ± 18.9181 | 0.6741 | 140.7479 ± 17.2418 | 2.4887 ± 0.4763 | 0.0177 |
| All layers averaged | 109.8458 ± 24.2337 | 105.1894 ± 29.8734 | 0.6404 | 130.7871 ± 26.6441 | 2.1307 ± 0.6396 | 0.0162 |
PSD95 and SAP102 puncta were imaged with SDM. The total densities of puncta were calculated from the densities of PSD95 and SAP102 puncta, together with the colocalization index (see text for details). The densities of asymmetric synapses (AS) were obtained from volumes of tissue reconstructed from serial sections using FIB-SEM. For each layer, the conversion factors is the quotient between the density of synapses obtained by FIB-SEM and the total density of puncta calculated from SDM images. Densities are given as average ± SD.