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. 2020 Jul 6;10(3):431–441. doi: 10.1007/s13534-020-00161-w

Table 1.

Forward and reverse primers used for RT-qPCR reactions

Gene Forward primer (5′–3′) Reverse primer (5′–3′) Annealing temperature (°C)
OCN CTG ACA AAG CCT TCA TGT CCA A GCG CCG GAG TCT GTT CAC TA 55.9
ALP TTT AGT ACT GGC CAT CGG CA ATT GCC CTG AGT GGT GTT GCA 57.9
GAPDH GAC ATG CCG CCT GGA GAA AC AGC CCA GGA TGC CCT TTA GT 58