Table 1.
Gene | Forward primer (5′–3′) | Reverse primer (5′–3′) | Annealing temperature (°C) |
---|---|---|---|
OCN | CTG ACA AAG CCT TCA TGT CCA A | GCG CCG GAG TCT GTT CAC TA | 55.9 |
ALP | TTT AGT ACT GGC CAT CGG CA | ATT GCC CTG AGT GGT GTT GCA | 57.9 |
GAPDH | GAC ATG CCG CCT GGA GAA AC | AGC CCA GGA TGC CCT TTA GT | 58 |