TABLE 3.
Detailed susceptibility pattern and STRAf genotyping of all Aspergillus fumigatus isolates from patients harboring isolates with decreased azole susceptibility.
| Patient ID | MIC (mg/L) |
Cyp51A profile | Sample date | Genotype |
||||||||||
| ITR | POS | VOR | 2A | 2B | 2C | 3A | 3B | 3C | 4A | 4B | 4C | |||
| AUH-1 | 1 | 0.25 | 2 | Wild-type | 15-05-2018 | 26 | 20 | 8 | 36 | 9 | 6 | 8 | 10 | 20 |
| AUH-2 | >16 | 1 | 4 | TR34/L98H | 10-01-2018 | 10 | 20 | 10 | 29 | 9 | 6 | 8 | 10 | 19 |
| >16 | 1 | 8 | TR34/L98H | 04-04-2018 | 23 | 21 | 16 | 65 | 9 | 9 | 10 | 24 | 21 | |
| >16b | 0.5 | 4 | TR34/L98H | 03-05-2018 | 23 | 21 | 16 | 65 | 9 | 9 | 10 | 24 | 21 | |
| RH-1 | 1 | 0.25 | 2 | Wild-type | 22-06-2018 | 23 | 23 | 15 | 23 | 11 | 19 | 13 | 9 | 5 |
| 2 | 0.25 | 2 | Wild-type | 17-08-2018 | 23 | 23 | 15 | 23 | 11 | 19 | 13 | 9 | 5 | |
| RH-2 | >16 | 2 | 2 | Wild-type | 08-02-2018 | 17 | 12 | 14 | 34 | 65 | 12 | 8 | 9 | 5 |
| S | S | S | NA, S | 15-03-2018 | 18 | 21 | 19 | 22 | 11 | 28 | 10 | 8 | 8 | |
| 2 | 0.25 | 2 | Wild-type | 19-04-2018 | 17 | 12 | 14 | 34 | 65 | 12 | 8 | 9 | 5 | |
| S | S | S | NA, S | 19-04-2018 | 18 | 21 | 19 | 22 | 11 | 28 | 10 | 8 | 8 | |
| S | S | S | NA, S | 18-05-2018 | 18 | 21 | 19 | 22 | 11 | 28 | 10 | 8 | 8 | |
| 2 | 1 | 1 | Wild-type | 07-06-2018 | 17 | 12 | 14 | 34 | 65 | 12 | 8 | 9 | 5 | |
| S | S | S | NA, S | 29-06-2018 | 18 | 21 | 19 | 22 | 11 | 28 | 10 | 8 | 8 | |
| S | S | S | NA, S | 16-08-2018 | 17 | 12 | 14 | 34 | 65 | 12 | 8 | 9 | 5 | |
| S | S | S | NA, S | 16-08-2018 | 18 | 21 | 19 | 22 | 11 | 28 | 10 | 8 | 8 | |
| 0.5 | 0.125 | 2 | Wild-type | 20-09-2018 | 18 | 21 | 19 | 22 | 11 | 28 | 10 | 8 | 8 | |
| 0.5 | 0.125 | 3 | Wild-type | 20-09-2018 | 19 | 24 | 20 | 36 | 11 | 17 | 16 | 11 | 8 | |
| RH-3 | 8 | 0.25 | 2 | Wild-type | 11-05-2018 | 21 | 25 | 18 | 27 | 12 | 6 | 21 | 10 | 8 |
| RH-4 | >16 | 4 | 8 | TR34/L98H/S297T/F495I | 26-04-2018 | 14 | 20 | 8 | 40 | 9 | 10 | 8 | 10 | 20 |
| >16 | 2 | 4 | TR34/L98H/S297T/F495I | 20-07-2018 | 14 | 20 | 8 | 40 | 9 | 10 | 8 | 10 | 20 | |
| 0.25 | 0.06 | 0.5 | Wild-type | 23-08-2018 | 14 | 20 | 8 | 36 | 9 | 9 | 8 | 10 | 20 | |
| RH-5 | NA | NA | NA | Wild-type | 11-07-2009 | 25 | 20 | 8 | 10 | 10 | 21 | 9 | 10 | 5 |
| S | S | S | NA, S | 27-01-2010 | NA | NA | NA | NA | NA | NA | NA | NA | NA | |
| >16 | 1 | 4 | TR343/L98H | 25-05-2017 | 23 | 21 | 16 | 97 | 9 | 6 | 5 | 9 | 10 | |
| >16 | 1 | 8 | TR343/L98H | 31-05-2017 | 23 | 21 | 16 | 96 | 9 | 6 | 5 | 9 | 10 | |
| >16 | 1 | 4 | TR343/L98H | 16-01-2018 | 23 | 21 | 16 | 98 | 9 | 6 | 5 | 9 | 10 | |
| >16 | 1 | 4 | TR34/L98H | 13-02-2018 | 23 | 21 | 16 | 95 | 9 | 6 | 5 | 9 | 10 | |
| >16 | 1 | 4 | TR343/L98H | 13-02-2018 | 23 | 21 | 16 | 99 | 9 | 6 | 5 | 9 | 10 | |
| >16 | 1 | 8 | TR343/L98H | 01-03-2018 | 23 | 21 | 16 | 101 | 9 | 6 | 5 | 9 | 10 | |
| RH-6 | >16 | 1 | 8 | TR34/L98H | 21-06-2018 | 14 | 20 | 17 | 31 | 11 | 9 | 8 | 14 | 20 |
| >16 | 1 | 8 | TR34/L98H | 01-10-2018 | 14 | 20 | 17 | 31 | 11 | 9 | 8 | 14 | 20 | |
| RH-7 | 0.25 | 0.06 | 0.5 | NA, S | 17-09-2018 | 14 | 18 | 12 | 34 | 9 | 7 | 8 | 10 | 21 |
| 0.125 | 0.06 | 0.5 | NA, S | 06-09-2018 | 18 | 20 | 9 | 26 | 10 | 6 | 8 | 10 | 5 | |
| 8 | 0.5 | 4 | TR34/L98H | 10-05-2018 | 23 | 21 | 16 | 97 | 9 | 6 | 5 | 9 | 10 | |
| >16 | 0.5 | 4 | TR34/L98H | 05-07-2018 | 23 | 21 | 16 | 99 | 9 | 6 | 5 | 9 | 10 | |
| RH-8 | >16 | >4 | 2 | M220R | 06-02-2018 | 23 | 23 | 15 | 40 | 11 | 46 | 10 | 26 | 8 |
| >16 | 4 | 1 | NA, R | 27-03-2018 | 23 | 23 | 15 | 40 | 11 | 46 | 10 | 26 | 8 | |
| >16 | >4 | 2 | Mixed, M220R and Wild-type | 03-04-2018 | 23 | 23 | 15 | 40 | 11 | 46 | 10 | 26 | 8 | |
| >16 | >4 | 2 | Mixed, M220R and Wild-type | 03-04-2018 | 23 | 23 | 15 | 41 | 11 | 46 | 10 | 26 | 8 | |
| >16 | 2 | 2 | Wild-type | 04-09-2018 | 23 | 23 | 15 | 40 | 11 | 45 | 10 | 26 | 8 | |
| 0.5 | 0.25 | 1 | Wild-type | 20-09-2018 | 23 | 23 | 15 | 37 | 11 | 42 | 10 | 26 | 8 | |
| RH-9 | >16 | 1 | 1 | M220K | 07-03-2018 | 18 | 19 | 11 | 27 | 10 | 37 | 20 | 11 | 5 |
| 0.5 | 0.125 | 0.5 | NA, S | 27-08-2018 | 18 | 28 | 8 | 29 | 11 | 17 | 8 | 8 | 5 | |
| RH-10 | >16 | 2 | 1 | M220K | 04-03-2018 | 11 | 12 | 11 | 17 | 9 | 15 | 8 | 8 | 5 |
| S | S | S | NA, S | 03-05-2018 | 18 | 23 | 26 | 22 | 11 | 25 | 22 | 10 | 8 | |
aAUH, Aarhus University Hospital, RH, Rigshospitalet. ITR, Itraconazole, POS, Posaconazole, VOR, Voriconazole, S, Susceptible, R, Resistant, NA, Not analyzed. bDuplicate isolates and isolates outside the study period are shown in gray.