TABLE 3.
Primer information on the microbiota for qPCR.
Target species | Primer sequence (5→3) | Tm (°C) |
Total bacteria | F: CGGYCCAGACTCCTACGGG | 60.0 |
R: TTACCGCGGCTGCTGGCAC | ||
Firmicutes | F: GGAGYATGTGGTTTAATTCGAAGCA | 64.5 |
R: AGCTGACGACAACCATGCAC | ||
Bacteroidetes | F: GGARCATGTGGTTTAATTCGATGAT | 60.0 |
R: AGCTGACGACAACCATGCAG | ||
Lactobacillus spp. | F: AGCAGTAGGGAATCTTCCA | 64.5 |
R: CACCGCTACACATGGAG | ||
Enterobacteriaceae | F: CATTGACGTTACCCGCAGAAGAAGC | 62.5 |
R: CTCTACGAGACTCAAGCTTGC | ||
L. johnsonii | F:CACTAGACGCATGTCTAGAG | 61.4 |
R:AGTCTCTCAACTCGGCTATG |