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. 2020 Aug 13;11:1941. doi: 10.3389/fmicb.2020.01941

TABLE 3.

Primer information on the microbiota for qPCR.

Target species Primer sequence (5→3) Tm (°C)
Total bacteria F: CGGYCCAGACTCCTACGGG 60.0
R: TTACCGCGGCTGCTGGCAC
Firmicutes F: GGAGYATGTGGTTTAATTCGAAGCA 64.5
R: AGCTGACGACAACCATGCAC
Bacteroidetes F: GGARCATGTGGTTTAATTCGATGAT 60.0
R: AGCTGACGACAACCATGCAG
Lactobacillus spp. F: AGCAGTAGGGAATCTTCCA 64.5
R: CACCGCTACACATGGAG
Enterobacteriaceae F: CATTGACGTTACCCGCAGAAGAAGC 62.5
R: CTCTACGAGACTCAAGCTTGC
L. johnsonii F:CACTAGACGCATGTCTAGAG 61.4
R:AGTCTCTCAACTCGGCTATG