Table 1.
Specifications and PCR conditions of the four commercial kits used for SARS-CoV-2 RNA detection.
| Kit name, manufacturer | PCR equipment | Target genes/No. tubes | Internal control | Volume of RNA eluates per test (μL) | No. amplification cycles | Running time of PCR | Cutoff Ct |
|---|---|---|---|---|---|---|---|
| Allplex 2019-nCoV, Seegene | CFX96 | E, RdRp, N/one | Bacteriophage MS2, spiked into sample | 8 | 45 | 110 min | <40 |
| PowerChek 2019-nCoV Real-time PCR, Kogene Biotech | CFX96, ABI7500, Gentier96 | E, RdRp/two | Recombinant DNA plasmid spiked into PCR mixture | 5 | 40 | 120 min | ≤35 |
| Real-Q 2019-nCoV Detection, BioSewoom | CFX96, ABI7500 | E, RdRp/one | Human RNase P, intrinsic | 5 | 40 | 110 min | Target gene: <38, Internal control: ≤35 |
| StandardM nCoV Real-Time Detection, SD Biosensor | CFX96, ABI7500, LC480 | E, RdRp/one | Pseudovirus particle, spiking of 5 μL into sample or 0.5 μL into PCR mixture | 10 | 45, including 5 pre-amplifications | 90 min | Target gene: ≤36*, Internal control: ≤32* |
SARS-CoV-2, severe acute respiratory syndrome coronavirus 2; Ct, cycle threshold.
*
Pre-amplification was not counted for Ct.