Table 3.
Precision tests for four commercial kits.
| Run No. | Ct values of target genes | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Allplex | PowerChek | Real-Q | StandardM | ||||||||||
| Positive control | Negative control | Positive control | Negative control | Positive control | Negative control | Positive control | Negative control | ||||||
| E | RdRp | N | E | RdRp | E | RdRp | E | RdRp | |||||
| Run 1 | 19.9 | 20.7 | 19.2 | ND | 22.9 | 23.8 | ND | 27.0 | 27.6 | ND | 26.1 | 24.3 | ND |
| Run 2 | 20.4 | 20.3 | 19.5 | ND | 23.3 | 22.5 | ND | 27.1 | 27.8 | ND | 26.4 | 25.1 | ND |
| Run 3 | 20.0 | 20.5 | 19.3 | ND | 23.0 | 22.3 | ND | 27.3 | 27.8 | ND | 26.1 | 24.8 | ND |
| Run 4 | 19.8 | 20.0 | 18.5 | ND | 23.2 | 22.7 | ND | 27.2 | 27.9 | ND | 26.4 | 24.7 | ND |
| Run 5 | 19.9 | 20.1 | 18.5 | ND | 23.0 | 21.7 | ND | 27.3 | 27.9 | ND | 25.9 | 24.3 | ND |
| Mean | 20.0 | 20.3a | 19.0b | ND | 23.1 | 22.6a | ND | 27.2 | 27.8a | ND | 26.2 | 24.6a | ND |
| SD | 0.23 | 0.29 | 0.47 | 0.16 | 0.77 | 0.13 | 0.12 | 0.22 | 0.34 | ||||
| CV (%) | 1.17 | 1.41 | 2.47c | 0.71 | 3.4c | 0.48 | 0.44c | 0.83 | 1.39c | ||||
ND, not detected; SD, standard deviation; CV, coefficient of variation,
a
Comparison of Ct using the Mann Whitney U-test between E and RdRp showed significant difference in Real-Q (p = 0.008) and StandardM (p = 0.008),
b
Ct of N was shorter than E and RdRp in comparison using Kruskall Wallis test of three target genes of Allplex (p = 0.004),
c
Comparison of the CV using F-test between target genes within the kits showed that RdRp is bigger than E in PowerChek (p = 0.005).