Table 4.

Reproducibility test using Allplex 2019-nCoV Assay.

Specimen No.	Type of specimen	Ct values of target genes and internal control
		1st test				2nd test				Test with 1:10 dilution
			E	RdRp	N		E	RdRp	N		E	RdRp	N
A0229st	Stool	I	36.8	–	–	N	–	–	–
A0229ur	Urine	I	–	39.8	–	I	–	–	39.3
A0229np	NPS	P	32.5	34.1	35.2	P	32.1	33.7	35.2	P	35.0	35.7	38.2
A0301np	NPS	P	25.4	26.3	28.8	P	25.2	26.7	28.9	P	28.7	30.8	32.5
A0303np	NPS	P	34.6	36.6	36.1	P	33.0	34.0	36.2	I	–	–	37.6
A0304np	NPS	P	25.3	27.3	28.8	P	25.7	27.1	28.8	P	28.9	31.0	32.1
A0305np	NPS	P	35.2	37.2	35.8	N	–	–	–
A0306np	NPS	N	–	–	–	N	–	–	–
A0307np	NPS	I	33.0	–	37.7	I	33.9	–	37.0
A0309np	NPS	N	–	–	–	N	–	–	–
A0310np	NPS	P	35.8	38.2	38.2	I	–	–	37.3
A0229sp	Sputum	P	16.1	17.2	19.0	P	16.4	18.0	20.2	P	19.9	21.8	23.8
A0301sp	Sputum	P	24.7	28.7	27.6	P	24.4	26.0	27.6	P	27.8	29.7	31.1
A0303sp	Sputum	P	26.1	29.5	29.4	P	27.1	29.1	29.5	P	30.3	32.9	32.6
A0304sp	Sputum	P	29.8	31.4	31.4	P	30.1	31.6	31.9	P	33.2	34.1	34.9
A0305sp	Sputum	P	32.4	34.3	35.0	P	31.2	34.3	34.6
A0306sp	Sputum	P	33.6	34.6	35.7	P	33.5	36.2	35.5
A0307sp	Sputum	I	–	36.8	–	P	34.3	35.0	36.9
A0308sp	Sputum	P	33.4	36.8	39.8	I	33.5	–	39.5
A0309sp	Sputum	I	36.0	–	38.6	P	34.4	38.7	36.9
A0310sp	Sputum	I	37.1	–	–	N	–	–	–
B0308np	NPS	P	19.9	22.0	23.0					P	24.1	25.9	27.0
B0309np	NPS	P	19.1	24.7	23.8					P	22.7	24.9	26.1
B0310np	NPS	P	22.6	24.5	26.8					P	26.6	29.0	29.5
B0308sp	Sputum	P	17.6	19.4	20.1					P	21.1	23.0	23.7
B0309sp	Sputum	P	23.6	25.6	27.3					P	28.0	30.3	31.2
B0310sp	Sputum	P	26.0	28.0	30.0					P	31.0	32.9	34.6