Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Aug 19;11(8):660. doi: 10.1038/s41419-020-02877-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 6 — a Predicted binding sites of miR-141 in cyclin-D1 and cyclin-D2 sequences. b RIP assay using Ago2 and IgG antibody (control) showing fold enrichment of miR-141, cyclin-D1, cyclin-D2, and CDKN2B-AS1 to Ago2 compared IgG controls in miR-141 overexpressing RCC cells. c Luciferase assays showing decreased reporter activity after co-transfection with either wild-type (WT), off-target (OT) cyclin-D2 or luciferase control constructs (EV) with miR-CON/miR-141 in ACHN and Caki1 cells. d, e Relative mRNA expression of cyclin-D1 and cyclin-D2 in both cell lines after miR-141 overexpression and CDKN2B-AS1 knockdown respectively. f Immunoblots showing changes in cyclin-D2 protein after CDKN2B-AS1 knockdown in ACHN and Caki1 cells. g Western blot analysis showing protein levels of cyclin-D1, cyclin-D2, pPaxillin, rac1, and β-actin (control) in ACHN and Caki1 cells overexpressing miR-141. h–j Immunostaining of cyclin-D1, rac1 (green) and pPaxillin (red) counterstained with DAPI (blue) in ACHN and Caki1 cells after transfecting with si-CON/CDKN2B-AS1-si2, scale bar: 2 mm (right bottom).