Table 1.

Inhibition constants for bedaquiline and oligomycin.

IC50 (μM) ± SEM
ATP hydrolysis				ATP synthesis
Purified yeast F1Fo		Yeast SMP		Yeast mitochondria
BDQ	Oligomycin A	BDQ	Oligomycin A	BDQ	Oligomycin A
0.027 ± 0.0025	0.107 ± 0.0011	1.28 ± 0.25	1.1 ± 0.04	1.1 ± 0.07	0.1 ± 0.008
				Human mitoplasts
				0.66 ± 0.05	0.0054 ± 0.0006

IC₅₀ for ATP hydrolysis and ATP synthesis for BDQ and oligomycin on yeast and human ATP synthase. Cyanide sensitive ATP synthesis for yeast mitochondria was 51.3 ± 2.6 nmoles/ATP/min/mg protein and was inhibited 98% with oligomycin (1 μM). Cyanide sensitive ATP synthesis for human mitoplasts was 9.0 ± 0.6 nmoles/ATP/min/mg protein and was inhibited 98% with oligomycin (1.25 μM). Total ATP synthesis for yeast mitochondria was inhibited by 98.6% with 1.5 mM sodium cyanide and by 59.2% with human mitoplasts.