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. 2020 Aug 19;3:453. doi: 10.1038/s42003-020-01181-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a The number of genes that significantly change gene expression (mRNA level) between non-stem cells and stem cells; orange indicates the number of genes that increase expression and blue are the number of genes that decrease expression compared with stem (padj < 0.01 + minimum mean 50 counts). b Auto-scaled heatmap showing gene expression and unsupervised clustering of the top 200 most variably expressed genes. c Gene expression heatmap and unsupervised clustering of n = 16 genes that are significantly enriched in stem cells compared to all non-stem cells (padj < 0.01+ minimum mean 50 counts). d Examples of stem enriched markers showing both mRNA expression paired with protein expression. Star annotation by cell type symbolizes significant differential mRNA expression compared with stem (padj < 0.01). e Unsupervised clustering of genes that significantly increase in expression from stem to both SecPDG and AbsPro (8-fold change cutoff, padj < 0.01 + minimum mean count 50 counts). Upper panel: the highly expressed Fcgbp gene is reported on a separate color scale. f, g Enrichr (Mouse Gene Atlas) and Panther (cellular component analysis, molecular function, and panther protein class) gene ontology analysis of n = 107 genes that are significantly higher in expression in all non-stem cell types compared with stem cells. FDR significance is defined by: *<0.05, **<0.01, ***<0.005, ****<0.001, and analysis was performed with the following biological replicate numbers: stem = 3, AbsPro = 3, SecPDG = 4, tuft = 5, Ent = 5, and EEC = 2.