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. 2020 Aug 19;3:453. doi: 10.1038/s42003-020-01181-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Gene ontology analysis (Panther) was performed on alternatively spliced (FDR < 0.05) and polyadenylated genes (padj < 0.05) with processing events specific to either AbsPro (red) or SecPDG (blue). Events are unique and not part of the overlap shown in Fig. 4b. b Two examples of alternatively spliced genes: Eif4a2, specific to SecPDG, and Cbfa2t2, specific to AbsPro. The exon inclusion rate for each event is shown in the bar graph. Eif4a2 was detected by MS and the abundance compared to mRNA is displayed (Cbfa2t2 protein was not detected). c Two examples of alternatively polyadenylated genes: Rbm3, AbsPro specific, and Ihh, specific to SecPDG. The percentage of distal polyA usage for each of the events is overlaid on the crypt-base diagram. FDR significance is defined by: *<0.05, **<0.01, ***<0.005, ****<0.001.