Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Aug 19;3:453. doi: 10.1038/s42003-020-01181-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 6 — a mRNA expression of Notch ligands (elevated in SecPDG/tuft), receptors (elevated in stem), and downstream targets. b mRNA expression of unfolded protein response (UPR) components including sensors, modulators, and downstream targets are elevated in SecPDG. Xbp1 activation, determined by a cytoplasmic splicing event, is elevated in SecPDG (inset—unpaired two-sided t test). Modulator Ndrg1 was detected via MS and shows protein is elevated in SecPDG (inset graph) consistent with mRNA expression (Ndrg1 protein was not detected in Stem). c mRNA expression of Fgf signaling components including ligands (showing some EEC expression), ligand enhancer Fgfbp1 expressed in AbsPro and Ent, receptors (well expressed in all cells, highest in SecPDG and tuft) and target gene (highly expressed in SecPDG and tuft). d Prostaglandin and leukotriene precursors and final products are produced by tuft cells (Supplementary Fig. 25), but absorptive lineage cells AbsPro and Ent, might contribute to the production (Plcb3, Dpep1, and Lta4h) and degradation (Slco2a1 and Hpgd) of prostaglandin signals. Prostaglandin receptor Ptger4 is enriched in SecPDG, whereas alternate receptors Pparg and Ppara are enriched in the absorptive lineage. Star annotation by gene name symbolizes significant differential mRNA expression in at least one cell type compared with stem (padj < 0.01). mRNA expression values are normalized counts and error bars are standard deviation. mRNA differential expression analysis was performed with the following biological replicate numbers: stem = 3, AbsPro = 3, SecPDG = 4, tuft = 5, Ent = 5, and EEC = 2.