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. 2020 Aug 19;11:4157. doi: 10.1038/s41467-020-17709-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Petri plates with a plastic divider create two chambers. The left chamber is poured with media without antibiotic, and the right chamber with antibiotic. After the media is set, the two chambers are connected by a thin layer of agar on the top of the bridge¹². Bacteria are inoculated in the left chamber as indicated, and allowed to swarm to the right chamber. b As in (a), but with dead bacteria layered on the surface of media on right.