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. 2020 Aug 19;9:142. doi: 10.1038/s41377-020-00379-4

Fig. 1. Optical setup and working principle of Wolf phase tomography (WPT).

Fig. 1

a WPT optical setup. The spatial light interference microscopy (SLIM) add-on module is mounted to the output camera port of a phase-contrast microscope. This module shifts the phase between the incident and scattered field with π/2 increments using the SLM. The interference patterns are recorded by the camera. b Spectrum, c autocorrelation, and d second-order derivative of the autocorrelation of the halogen source measured by the spectrometer. e Three phase-shifted frames of 2-μm polystyrene beads are acquired using the SLIM module (×63/1.4 NA)