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. 2020 Aug 19;10:13988. doi: 10.1038/s41598-020-70871-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) mRNA levels of GLI1 and indicated genes, expressed in arbitrary units, in Colo205 treated with increasing doses of 5-FU and Oxaliplatin (5-FU + Ox), starting from 1 μM to 10 μM and 10 μM GANT61 during 5 weeks. *p < 0.05 versus starting values (two-way ANOVA test). (B) mRNA levels of GLI1 and indicated genes, expressed in arbitrary units, in HCT15 treated with increasing doses of 5-FU + Ox, starting from 1 to 10 μM and 10 μM GANT61 during 5 weeks. *p < 0.05 versus starting values; ^§p < 0.05 5-FU + Ox versus 5-FU + Ox + GANT61 (two-way ANOVA test). Data are representative of three independent experiments, (A, B). Histograms were created using GraphPad Prism version 6.0 for macOS, https://www.graphpad.com.