FIGURE 5.

Rofecoxib treatment decreased the activity of glial cells, which resulted in increased numbers of motor neurons. (A,B) Western blot analysis was employed to detect the protein levels of GFAP and Iba1. The optical densities of GFAP and Iba1 were analyzed with ImageJ software. (C,D,F) IHC was used to detect the morphology and integrated density of astrocytes and microglia in the lumbar anterior horns of SOD1^G93A mice. (E,G) Spinal cords were collected for Nissl staining. The numbers of motor neurons were calculated and analyzed by Image-Pro Plus (IPP). The data represent the means ± SEs from independent experiments. *p < 0.05; **p < 0.01, compared with non-transgenic mice. ^#p < 0.05, ^##p < 0.01, compared with vehicle-treated SOD1^G93A mice.