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. 2020 Aug 13;11:1264. doi: 10.3389/fphar.2020.01264

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Copyright © 2020 Luo, Song, Wang, Huang, Liu, Wang, Hong and Yuan

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cryptotanshinone (CPT) inhibits the proliferation of Huh7 and MHCC97-H cells. (A) 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) analysis of human hepatocellular carcinoma (HCC) cell lines, including Huh7 and MHCC97-H cells, treated with the indicated doses of CPT for 24 and 48 h. These experiments were independently repeated three times. (B) A colony formation assay was performed for Huh7 and MHCC97-H cells incubated with various doses (0, 3, 6, and 12 μM) of CPT. Colony survival relative to control groups was calculated and presented as mean ± standard deviation (SD) (n = 3). (C) Proliferative capacity was measured by EdU assay in Huh7 and MHCC97-H cells treated with the indicated concentrations of CPT (0, 3, 6, and 12 μM) for 24 h. *P < 0.05, **P < 0.01, and ***P < 0.001 versus the control group.