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Nuclear OPN expression in explanted hearts but not endomyocardial biopsies (EMBs). In Patient #2, nuclear OPN (shown in white) in ACTA1-positive cardiomyocytes (shown in red) is visible in failing native and transplanted hearts, but not in the serial follow-up EMB sample collected at almost 1 week post first transplant. Representative confocal z-stack images are shown. Scale bar = 20 μm.
