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. 2020 Aug 13;11:928. doi: 10.3389/fphys.2020.00928

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Copyright © 2020 Irion, Dunkley, John-Williams, Condor Capcha, Shehadeh, Pinto, Loebe, Webster, Brozzi and Shehadeh.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Nuclear OPN expression in explanted hearts but not endomyocardial biopsies (EMBs). In Patient #3, nuclear OPN (shown in white) in ACTA1-positive cardiomyocytes (shown in red) is visible in failing native and transplanted hearts, but in none of the three serial follow-up EMB samples collected at 1 week, 1 month, and 1 year post first transplant. Representative confocal z-stack images are shown. Scale bar = 20 μm.