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Robustness and repeatability of the droplet digital PCR assay for porcine epidemic diarrhea virus.
| Concentration of PUC57-N plasmid (copies/μL) | Intra-assay |
Inter-assay |
||||||
|---|---|---|---|---|---|---|---|---|
| Viral load of 3 tests (copies/μL) | CV% | Viral load of 3 tests (copies/μL) | CV% | |||||
| 4 × 103 | 23.3 | 22.6 | 23.9 | 2.80 | 20 | 22 | 18 | 10.7 |
| 4 × 104 | 226 | 241 | 262 | 7.44 | 230 | 239 | 297 | 14.4 |
| 4 × 105 | 2,370 | 2,510 | 2,380 | 3.29 | 2,120 | 2,250 | 2,500 | 8.5 |
