. 2020 Aug 13;14:770. doi: 10.3389/fnins.2020.00770

TABLE 3.

Standardized mRNA expression of genes affected by dietary treatment¹.

Measure³	CON	BMOS	HMO	BMOS + HMO	Pooled	P-Value²
					SEM	BMOS	HMO	INT
5HTR1⁴	0^a	−0.49^a	−0.69^a	0.06^a	0.31	0.654	0.808	0.039
CHRM3	0^ab	−0.59^ab	−0.70^b	0.39^a	0.29	0.366	0.612	0.004
GABRB2	0^ab	−0.31^b	−0.30^b	0.99^a	0.28	0.071	0.064	0.004
GABRR1⁴	0^a	−0.12^a	−0.70^a	−0.61^a	0.31	0.959	0.047	0.724
GLRA4⁵	0^ab	−0.45^b	−0.07^ab	0.70^a	0.32	0.548	0.048	0.027
GRIN1⁴	0^a	0.45^a	0.02^a	0.53^a	0.37	0.035	0.818	0.888
GRIN2D⁴	0^a	−0.62^a	−0.56^a	−0.05^a	0.34	0.822	0.979	0.037
HDAC5⁴	0^a	0.62^a	0.17^a	0.87^a	0.35	0.015	0.428	0.876
NCAM1⁴	0^a	−0.48^a	−0.50^a	0.21^a	0.33	0.682	0.743	0.041
NR4A2⁴	0^a	−0.37^a	−0.52^a	0.56^a	0.30	0.211	0.480	0.015
SLC17A6⁴	0^a	−0.58^a	−0.48^a	0.32^a	0.34	0.673	0.437	0.012
SLC18A2^4,5	0^a	−0.47^a	−0.99^a	−0.76^a	0.30	0.670	0.031	0.230
SLC1A7	0^a	−0.11^a	−0.14^a	−1.23^b	0.32	0.026	0.020	0.066
SLC6A3^4,5	0^a	0.28^a	−0.57^a	−0.62^a	0.34	0.689	0.018	0.593
SNAP25⁴	0^a	−0.58^a	−0.54^a	0.32^a	0.30	0.615	0.536	0.015

¹Abbreviations: CON, control group; HMO, pigs fed human milk oligosaccharides; BMOS; pigs fed bovine milk oligosaccharides, BMOS + HMO, pigs fed both human and bovine milk oligosaccharides; SEM, standard error of the mean; INT, interaction effect of HMO and BMOS; 5HTR1, serotonin receptor 1; CHRM3, cholinergic receptor muscarinic 3; GABRB2, GABA type A receptor subunit β2; GABRR1, GABA type a receptor subunit ρ1; GLRA4, glycine receptor α4; GRIN1 and GRIN2D, glutamate ionotropic receptor NMDA type subunit 1 and subunit 2D; HDAC5, histone deacetylase 5; NCAM1, the neural cell adhesion molecule 1; NR4A2, nuclear receptor subfamily 4 group a member 2; SLC17A6, solute carrier family 17 member 6; SLC18A2, solute carrier family 18 member A2; SLC1A7, solute carrier family 1 member 7; SLC6A3, solute carrier family 6 member 3; SNAP25, synaptosome associated protein 25. ²Data analyzed via two-way ANOVA with post hoc Tukey adjustment for multiple comparisons. ³Standardized values for mRNA expression (mean = 0, standard deviation = 1) centered by control group. Only measures significantly altered by diet are shown. ⁴Mean separation insignificant after Tukey adjustment. ⁵Number of samples below threshold (median of negative controls): GLRA4, 10; SLC18A2, 7; SLC6A3, 14. ^abcMeans without a common superscript differ (P < 0.05).