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. 2020 Aug 13;11:1939. doi: 10.3389/fmicb.2020.01939

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Copyright © 2020 Soutourina, Dubois, Monot, Shelyakin, Saujet, Boudry, Gelfand, Dupuy and Martin-Verstraete.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Growth and fermentation products of the WT and sigL::erm mutant strains. (A) Growth curves of the 630Δerm and the sigL::erm strains in TY and TY + 0.5% glucose. Growth curves are representative of at least three independent experiments. Concentrations of volatile (B) and non-volatile (C) fermentations end products of the 630Δerm and the sigL::erm strains grown 48 h in TY. Gas-liquid chromatography analysis was performed from the supernatant of the culture of both strains as described in materials and methods. The concentration of the fermentation end products was standardized on the OD_600nm of the cultures after 48 h of growth. We could not detect butanol nor ethanol by these assays. The error bars represent the standard deviation of the mean. Asterisks indicate statistical significance (t-test or t-test Welch, ^∗∗∗p ≤ 0.001, ^∗∗p ≤ 0.01, ^∗p ≤ 0.05).