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. 2020 Aug 7;11:770. doi: 10.3389/fgene.2020.00770

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Copyright © 2020 Wang, Liu, Wang, Ma, Zhang and Xu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The methylation levels of queen larvae and worker bee larvae at 3, 4, and 5 days old. (A) mC level. (B) mCpG level. The methylation levels of the single C site = 100 × The numbers of the reads supporting the methylated C site/The numbers of the reads supporting the methylated C site + The numbers of the reads supporting the un-methylated C site. Regional level of methylation (C) = 100 × The sum of methylation levels of all C site in this region/The numbers of C sites, whose depth was not less than 5. Regional level of methylation (CG) = 100 × The sum of methylation levels of all CG site in this region/The numbers of CG sites, whose depth was not less than 5.