Figure 1.

Evidence of LPG2 duplication in L. infantum. (A) Genomic DNA from L. infantum Hyg/NeoR strain was amplified by PCR using specific oligonucleotides (Supplementary Table 1) to verify LPG2 gene targeting by homologous recombination. (B) Comparison of the genomic contexts where LPG2 (magenta box) was originally located (in an unplaced 19 kbp contig shown at the bottom) to the recent resequencing and reassembly of the L. infantum JPCM5 genome (Gonzalez-de la Fuente et al., 2017), where the duplication of the LPG2 gene array is evidenced in chromosome 34 (top). Gray connecting segments indicate sequence conservation, and the coordinates at the top refer to the novel chromosome 34 assembly.