FIGURE 1.
CXCR4 mediates T-ALL cell migration toward CXCL12. (A,B) Starved cells were pretreated with 500 ng/ml AMD3100 or equal concentration of solvent on ice for 30 min, washed with medium, and 3 × 105 cells in 100 μl were used in the following transwell assay. The relative migration rate is shown. (C–F) Cells were infected with lentivirus containing CXCR4 shRNAs and control shRNA. Then, 72 h after infection, the interference efficiency of shRNAs to CXCR4 was detected by Western blot using CXCR4 antibody (C,D), and transwell assays were conducted (E,F). Each experiment was repeated at least three times. Western blot bands were analyzed using Image Pro Plus 6.0, and fold changes of CXCR4 were normalized according to ACTB. ACTB was used as the loading controls. *p < 0.05, **p < 0.01, ****p < 0.0001.