FIGURE 2.

RhoGDI2 negatively regulates T-ALL cell migration toward CXCL12. (A,B) shRNA to RhoGDI2 or control shRNA was transfected into HEK293T to generate lentivirus, JURKAT or CCRF-CEM cells were infected, and the interference efficiency was detected by Western blot, using RhoGDI2 antibody. (C,D) shRNA that successfully downregulated RhoGDI2 was used in the transwell assay. Each experiment was repeated at least three times. Western blot bands were analyzed using Image Pro Plus 6.0, and fold changes of RhoGDI2 were normalized according to ACTB. ACTB was used as the loading controls. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.