FIGURE 3.

Phosphorylation of RhoGDI2 Tyr 24, 130, or 153 recovered CXCL12-mediated ALL migration. JURKAT (A) or CCRF-CEM (B) cells were infected with lentivirus containing nucleotides expressing GFP-RhoGDI2-WT, Y24E, Y130E, or Y153E, and the overexpression levels of WT or mutated RhoGDI2 were detected 48 h after infection by Western blot, using GFP antibody. (C,D) Transfected cells were used in the transwell assay. Each experiment was repeated at least three times. Western blot bands were analyzed using Image Pro Plus 6.0, and fold changes of GFP, GFP-RhoGDI2-WT, or mutants were normalized according to ACTB. ACTB was used as the loading controls. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.