FIGURE 6.

Knockdown expression of Src by siRNAs inhibited CXCR4-mediated T-ALL migration toward CXCL12. (A,B) siRNAs were transfected into JURKAT or CCRF-CEM cells using the methods described in the materials and methods section; 48 h after transfection, cell lysates were prepared for Western blot assay to detect the interference efficiency of siRNAs on Src. (C,D) Transfected cells were used in the transwell assay. Each experiment was repeated at least three times. Western blot bands were analyzed using Image Pro Plus 6.0, and fold changes of Src were normalized according to ACTB. ACTB was used as the loading controls. *p < 0.05, **p < 0.01, comparing with the negative control (CXCL12−) group. ^#p < 0.05 comparing with the positive control (CXCL12+) group.