FIGURE 8.

Lck was activated by CXCL12 and knocked down the expression of Lck by siRNA-inhibited CXCR4-mediated T-ALL migration toward CXCL12. (A,B) Starved JURKAT or CCRF-CEM cells were incubated with CXCL12, and then cell lysates were prepared: 60 μg of cell lysates were subjected to SDS-PAGE and Western blot assay, using active Lck antibody, Lck (394 Tyr-P). Western blot bands were analyzed using Image Pro Plus 6.0, and fold changes of active Lck were normalized according to total Lck. Total ABL1 was used as another loading control. (C,D) siRNAs were transfected into JURKAT or CCRF-CEM cells using the methods described in the materials and methods section; 48 h after transfection, cells were used in the transwell assay. Each experiment was repeated at least three times, and three repeats were included each time. **p < 0.01 comparing with the negative control (CXCL12−) group. ^##p < 0.01 comparing with the positive control (CXCL12+) group.