FIGURE 9.

Knockdown expression of ABL1 by siRNAs inhibited CXCR4-mediated T-ALL migration toward CXCL12. (A,B) siRNAs were transfected into JURKAT or CCRF-CEM cells using the methods described in the materials and methods section; 48 h after transfection, cell lysates were prepared for Western blot assay to detect the interference efficiency of siRNAs on ABL1. Western blot bands were analyzed using Image Pro Plus 6.0, and fold changes of ABL1 were normalized according the loading control ACTB. (C,D) Transfected cells were used in the transwell assay. Each experiment was repeated at least three times. *p < 0.05, ***p < 0.001, comparing with the negative control (CXCL12−) group. ^#p < 0.05, ^###p < 0.001, comparing with the positive control (CXCL12+) group.