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. 2020 Aug 6;8:921. doi: 10.3389/fbioe.2020.00921

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Copyright © 2020 Chu, Hsieh and Wu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) microscopic observations of the first-step ODEP cell manipulation process; (I) a static rectangular light bar (L: 3.9 mm, W: 100 μm) with a particular angle (e.g., 15°) to the flow direction of the cell suspension was designed in the main microchannel, (II–VI) the SW620 cancer cells (indicated by arrows) were sorted, separated, and then guided along the rectangular light bar image to the side microchannel for collection (a video clip is provided as Supplementary Videos 1, 2), (B) the purity (%) of SW620 cancer cells obtained after the first and second step ODEP cell manipulation processes for the prepared cell samples originally containing 5 and 10% SW620 cancer cells, respectively, (C) microscopic observations of the second-step ODEP cell manipulation process; (I) a certain amount of the cancer cells were collected in the side microchannel, (II) the liquid flow in the side microchannel was driven to flux the cells collected so that they were evenly spread within the side microchannel, (III) fluorescence microscopy observation was carried out to identify the species of the cells [i.e., fluorescence stained SW620 cancer cells (the red dots) and immunofluorescence stained magnetic microbead-bound Jurkat cells (the green dots)] collected in the side microchannel for positioning the SW620 cancer cells, (IV) static circular light images were illuminated on each SW620 cancer cell. Meanwhile, the side microchannel was illuminated with a rectangular light bar to manipulate the magnetic microbead-bound Jurkat cells into the side microchannel, in which O-ring-like non-illuminated patterns were designed as partitions to separate the light-illuminated SW620 cancer cells and the other cells, (V–VII) the rectangular light bar on the side microchannel was then moved to manipulate the magnetic microbead-bound Jurkat cells to remove them from the side microchannel, and this process was repeated for 5 times, (VII–IX) through the previous process, the cell purity of the SW620 cancer cells in the side microchannel was greatly improved (a video clip is provided as Supplementary Video 3), (D) microscopy observation of the cells collected in the side microchannel after two-step ODEP manipulation processes for the prepared cell samples originally containing 5 and 10% SW620 cancer cells, respectively (Bright field: the upper row, Fluorescence observation: the lower row, SW620 cancer cells: the red dots; 50 nm magnetic microbead-bound Jurkat cells: the green dots).