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The primer sequences for PCR.
| Target gene | Primer sequences |
|
| Forward primer | Reverse primer | |
| TIGIT | 5-CGTGAACGATACAGGGGAGT-3 | 5-GCAATGGAATCTGGAACCTG-3 |
| CD226 | 5-GGCAGAAATTTCACCTCCAA-3 | 5-GCAAGTAGCAGCGGTAAAGC-3 |
| PD-1 | 5-ACCTGGGTGTTGGGAGGGCA-3 | 5-GGAGTGGATAGGCCACGGCG-3 |
| CD155 | 5-TCCTGTGGACAAACCAATCA-3 | 5-GTTACGGGACATGCCTGAGT-3 |
| GAPDH | 5-GCACCGTCAAGGCTGAGAAC-3 | 5-TGGTGAAGACGCCAGTGGA-3 |
