Table 2 .
Reversal effect of three peptides on transfected ABCB1-overexpressing cells.
Treatment | IC50 1 (μM) (RF2) | |
---|---|---|
HEK293/pcDNA3.1 | HEK293/ABCB1 | |
Paclitaxel | 1.449 ± 0.121 (1.00) | 30.924 ± 4.665 (21.34) |
+ HX-12A (3 μM) | 1.405 ± 0.285 (0.97) | 22.117 ± 1.199 (15.26) |
+ HX-12B (3 μM) | 1.302 ± 0.255 (0.90) | 20.190 ± 1.047 (13.93) |
+ HX-12C (3 μM) | 1.446 ± 0.462 (1.00) | 1.501 ± 0.342*** (1.04) |
+ Verapamil (3 μM) | 1.486 ± 0.377 (1.03) | 1.708 ± 0.147*** (1.18) |
Doxorubicin | 1.235 ± 0.571 (1.01) | 31.233 ± 5.406 (25.29) |
+ HX-12A (3 μM) | 1.206 ± 0.405 (0.98) | 25.327 ± 4.318 (20.51) |
+ HX-12B (3 μM) | 1.221 ± 0.488 (0.99) | 22.374 ± 3.774 (18.12) |
+ HX-12C (3 μM) | 1.420 ± 0.453 (1.09) | 1.182 ± 0.253*** (0.94) |
+ Verapamil (3 μM) | 1.271 ± 0.358 (1.03) | 1.245 ± 0.265*** (1.00) |
Cisplatin | 2.214 ± 0.541 (1.00) | 2.665 ± 0.208 (1.20) |
+ HX-12A (3 μM) | 2.337 ± 0.438 (1.06) | 2.525 ± 0.284 (1.14) |
+ HX-12B (3 μM) | 2.403 ± 0.389 (1.09) | 2.692 ± 0.176 (1.22) |
+ HX-12C (3 μM) | 2.464 ± 0.144 (1.11) | 2.184 ± 0.317 (0.99) |
+ Verapamil (3 μM) | 2.128 ± 0.238 (0.96) | 2.287 ± 0.138 (1.03) |
1IC50 values are represented as mean ± SD of at least three independent experiments performed in triplicate. 2RF, resistant fold, calculated by the IC50 values in the transfected ABCB1-overexpressing cell line HEK293/ABCB1 divided by the IC50 values in the empty-vector transfected cell line HEK293/pcDNA3.1. ***represents p < 0.001, compared to the value of HEK293/ABCB1 control group.