Figure 5.

(A) Fluorescence microscope with Beclin1 Red Probe analysis and quantitative results of autophagy intensity in H9c2 cells treated with Control; HG; HG +H₂S; H₂S. (B) Quantitative results of BECLIN1 red fluorescence intensity in H9c2 treated with Control; HG; HG +H₂S; H₂S. Data were normalized to control. (C) Western blotting analysis the expression of Atg5, Atg12, Atg16L1 and Beclin1 in H9c2 cells treated with Control; HG; HG +H₂S; H₂S. n = 3; *p < 0.05 vs control, **p < 0.01 vs control, ***p < 0.001 vs control, ^#p < 0.05 vs HG, ^##p < 0.01vs HG, ^$p < 0.05 vs HG+H₂S; ^$$p < 0.01 vs HG+H2S; HG, high glucose; H₂S, hydrogen sulfide; PAG, dl-propargylglycine.