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. 2020 Aug 4;11:1828. doi: 10.3389/fmicb.2020.01828

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Copyright © 2020 Wang, Sun, Ren, Li, Yang and Zhou.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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RepA-dependent nucleolar exclusion of V2. RFP-H2B plant leaves were infiltrated with Agrobacterium tumefaciens cultures carrying constructs to express GFP-V2 and the other six individual ORFs (V1, V3, V4, V5, RepA, and Rep) of MMDaV. Fluorescence was visualized under a Zeiss LSM 880 confocal laser scanning microscope at 36 hpi. The corresponding region in the white box in column 1 is magnified and shown from Column 2 to Column 5. RFP-H2B was used as a nuclear marker. This experiment was done three times and more than 20 cells were observed per sample and replicate. Scale bars correspond to 10 μm.