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. Author manuscript; available in PMC: 2020 Aug 20.
Published in final edited form as: Cancer Res. 2009 Dec 15;69(24):9183–9191. doi: 10.1158/0008-5472.CAN-09-1016

Figure 4.

Figure 4.

Regulation of p21 transcription by WNT16B. A, qRT-PCR analysis of WNT16B and p53 expression in MRC5-hTERT cells transfected with a control siRNA (siCT) and a siRNA targeting WNT16B (siWNT16B) or p53 (siP53). B, MRC5-hTERT fibroblasts expressing siCT or siWNT16B and treated with H2O2 were stained for SA-β-Gal expression and quantified. Columns, mean; bars, SD. C, qRT-PCR analysis of p21 expression after H2O2 treatment (left) or X radiations (right). MRC5-hTERT cells were transfected with siCT, siWNT16B, or siP53. D, Western blot analysis of WNT16, p53, and p21 expression and p53 and AKT activation after X radiations. α-Tubulin was used as a loading control. The film was overexposed to detect WNT16 basal expression and evaluate the siWNT16B efficiency. Note the presence of a cross-reacting band seen only in hTERT-immortalized fibroblasts.