Regulation of AKT by WNT16B. A, Western blot analysis of AKT activation in dividing (PDL28), senescent (PDL64), WNT16Bkd, and p53kd MRC5 fibroblasts. α-Tubulin was used as a loading control. B, Western blot analysis of AKT activation and WNT16, p53, and p21 protein expression in pBabe and pBabe-WNT16B MRC5 fibroblasts. α-Tubulin was used as a loading control. C, analysis of AKT activation in pBabe-WNT16B MRC5 fibroblasts by Western blot after treatment with LY294002.