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. 2020 Aug 10;20(4):105. doi: 10.3892/ol.2020.11966

Figure 3.

Figure 3.

miR-326 silencing partially reverses the effects of RPPH1 knockdown. (A) Starbase prediction of the binding site between RPPH1 and miR-326. (B) miR-326 expression in A549 cells transfected with miR-326 mimic or inhibitor. (C) RPPH1 luciferase reporter assays were carried out in A549 cells transfected with miR-326 mimic or inhibitor. (D) miR-326 levels, (E) Invasion potential (magnification, ×100; scale bar, 50 µm) and (F) E-cadherin and vimentin protein levels in A549 cells transfected with shRPPH1 alone or with miR-326 inhibitor. (G) Cell Counting Kit-8 and (H) colony formation assays were performed to evaluate CDDP resistance of A549 cells transfected with RPPH1 overexpression vector alone or with miR mimic. *P<0.05, **P<0.01. miR, microRNA; RPPH1, ribonuclease P RNA component H1; CDDP, cisplatin/cis-diamminedichloroplatinum; sh, short hairpin; NC, negative control.