Figure 3.

Phloretin induced cell apoptosis and cell cycle arrest in PCa cells. (a, b) LNCaP and PC-3 cells were treated with phloretin (0, 20, 50, and 100 μM) for 24 h and then harvested for flow cytometry analysis to check cell cycle and apoptosis. Percentages of cell cycle phages and apoptosis were quantified, respectively. (c) LNCaP and PC-3 cells were treated with phloretin (0, 20, 50, and 100 μM) for 24 h, and then cells were stained by DAPI, observed, and photographed with fluorescence microscope under 40x magnification. (d) LNCaP and PC-3 cells were treated with phloretin (0, 20, 50, and 100 μM) and then harvested for western blot assay to check the protein levels of c-Caspase 3, c-PARP-1, c-Caspase 8, c-Caspase 9, Bcl-2, BAX, XIAP, p53, Cyclin B1, and β-actin (loading control).