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circCCDC66 expression was increased in OS tissues and cells. (a) qRT-PCR assay of circCCDC66 after treatment with RNase R. (b) qRT-PCR assay of CCDC66 mRNA in OS cells treated with RNase R. (c) The relative expression levels of circCCDC66 in 12 pairs of OS tissues and matched noncancerous tissues by qRT-PCR assay. (d) Detection of circCCDC66 expression levels in OS cell lines by qRT-PCR assay. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.
