Table 2.
Comparison of five different methods for the preparation of MEF from M. koenigii leaflets.
| Steps | Method 1 | Method 2 | Method 3 | Method 4 | Method 5 |
|---|---|---|---|---|---|
| Dry leaflets (starting material) | 50 grams | 50 grams | 50 grams | 50 grams | 50 grams |
| Infusion with water (50°C) | Water fraction was discarded | Water fraction was discarded | — | — | — |
| Maceration with solvent for (72 h) | Ethanol (water-extracted leaflet residue) | Ethanol : H2O (95 : 05) (water-extracted leaflet residue) | Ethanol : H2O (95 : 05) | Ethanol : H2O (90 : 10) | Ethanol : H2O (80 : 20) |
| Weight of extract (grams) (yield %) | 4.12 (8.24) | 4.00 (8.0) | 4.9 (9.8) | 6.0 (12) | 7.86 (15.72) |
| Mahanine (%) in extract by HPLC | 39.0 | 40.1 | 35.4 | 32.6 | 20.0 |
| Fractionation with ethyl acetate : water (3 : 1) Weight of fraction (final product in grams) |
3.92 | 3.66 | 3.24 | 3.25 | 2.91 |
| Yield (%) (amount of fraction/100 grams of dry leaflets) | 7.8 | 7.3 | 6.5 | 6.5 | 5.8 |
| Comparison of mahanine (%) in extract and fraction by HPLC | Enriched to 39.8 | Enriched to 45.0 | Enriched to 44.6 | Enriched to 43.3 | Enriched to 42.2 |
| Total amount of mahanine in final product (mg) | 1560 | 1640 | 1450 | 1410 | 1230 |
Five different methods showing the enrichment of the mahanine percent yield of MEF. This experiment was repeated at least thrice to confirm the results.