Figure 2.

Effects of elamipretide on the canonical nod-like receptor pyrin domain-containing 3 (NLRP3) inflammasome-caspase-1 pathway in the hippocampus in aged mice after surgery. (A) Representative western blotting and quantitative analysis of protein levels of NLRP3, Pro-caspase-1, and cleaved caspase-1 in the hippocampus in aged mice. (B) Representative images of caspase-1 Immunohistochemistry (IHC) staining in the hippocampal CA1 region. Cells with brownish-yellow cytoplasm are positive for caspase-1. Scale bar = 50 μm for all photographs. The lower panel presents the number of caspase-1-positive neurons in the CA1 region of the hippocampus. Values are presented as mean ± SEM (n = 6 mice/group). *p < 0.05 vs. the Veh group; ^#p < 0.05 vs. the Sur group.