. 2020 Aug 3;148:e172. doi: 10.1017/S0950268820001697

Table 1.

List of PCR primers used to amplify eight Cyclospora cayetanensis genotyping targets

Marker #	Genome	Target alias	Primer name	Primer Sequence (5′-3′)	Target	Amplicon length (bp)	Reference
1	Nuclear	CDC-1	GT1-F	CTCCTTGCTGCTCAGAACGA	ATP synthase	175	[11]
1	Nuclear	CDC-1	GT1-R	CAAGAGAGGAGCAGTGGCAA	ATP synthase	175
2	Nuclear	CDC-2	GT2-F	TGCAAACTACTAAGGGCGCA	U3 small nucleolar RNA-associated protein 11	246
2	Nuclear	CDC-2	GT2N-R	CGCCTTCTCTTGAGCCTTGA	U3 small nucleolar RNA-associated protein 11	246
3	Nuclear	CDC-3	GT3-F	AATCGAATCGGTGCAGTGCTTA	uncharacterised	220
3	Nuclear	CDC-3	GT3N-R	GACTGAACGTGTGAGAGGGG	uncharacterised	220
4	Nuclear	CDC-4	GT4-F	GTAGATGGGTCCTTGAAGGCT	ATP-dependent RNA helicase rrp3	179
4	Nuclear	CDC-4	GT4N-R	CAGACGCCTAAGGAACCGAA	ATP-dependent RNA helicase rrp3	179
5	Nuclear	HC378	HC378F	CCCCTGCCTTGTTCTTGGTGAA	Sec14 family protein	650	[9]
5	Nuclear	HC378	HC378R	CCGGCGACACAGAGGTACC	Sec14 family protein	650
6	Nuclear	HC360i2	HC360i2F	CCCATTACGCCGCATAGAGT	uncharacterised	469
6	Nuclear	HC360i2	HC360i2R	GCATTGCAAAGCCAGTCAGC	uncharacterised	469
7	Mitochondrial	Mt-Junction	cyclo_mit-100F	TACCAAAGCATCCATCTACAGC	Mitochondrial junction repeat	109 to 214	[10]
7	Mitochondrial	Mt-Junction	cyclo_mit-54R	CCCAAGCAATCGGATCGTGTT	Mitochondrial junction repeat	109 to 214	[10]
8	Mitochondrial	MSR	15F	GGACATGCAGTAACCTTTCCG	Mitochondrial rRNA	674	[9]
8	Mitochondrial	MSR	688R	AGGAAAGGTTAACCGCTGTCA	Mitochondrial rRNA	674	[9]