Figure 3.

Effect of X-5 on the cellular metabolic process and Xcn1-biosynthesis-related gene transcription of X. nematophila CB6. (A) Metabolic activity of X. nematophila CB6 in treatment and control measured by the 3-(4,5-dimethylthiazol-2-yl)–2,5-diphenyltetrazolium bromide (MTT) assay. (B) Reducing sugar concentration during fermentation was measured by the 3,5-dinitrosalicylic acid (DNS) method. (C) Relative transcription level of Xcn1-biosynthesis-related genes (xcnA, xcnB, xcnF, xcnH, xcnK, and ompR) was measured by real-time quantitative polymerase chain reaction (RT-qPCR), and all of the data were normalized based on the expression of recA. Data was shown as means ± standard errors (SEs).